Double Embedding in Cellulose Nitrate and Paraffin Wax, an Old and Useful Method that is Easily Misunderstood

An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...

Cell Shrinkage caused by Fixatives and Paraffin - wax Embedding in Ordinary Cytological Preparations

A series of measurements was made of the diameters of the cells and nuclei of the primary spermatocytes of the snail Helix aspersa in preparations made by embedding in paraffin wax after fixation in a number of different cytological fixatives. These were compared with similar measurements made on the living spermatocytes mounted in suitable saline solutions, and the shrinkage that the cells und...

A Rapid Method of Paraffin Embedding

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence.

AIMS To develop and immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. METHODS This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and in...